Volume 4, Issue 1, March 2018, Page: 25-29
Down-Regulation of Long Non-Coding RNA CASC9 Suppresses the Migration and Invasion of Esophageal Cancer Cells
Maliha Ghaffar, Beijing Key Laboratory of Environmental & Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, China
Sara Khodahemmati, Beijing Key Laboratory of Environmental & Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, China
Jintao Li, Beijing Key Laboratory of Environmental & Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, China
Muhammad Shahzad, School of Economics and Management, North China Electric Power University, Beijing, China
Minglian Wang, Beijing Key Laboratory of Environmental & Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, China
Yangjunqi Wang, Beijing Key Laboratory of Environmental & Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, China
Yi Zeng, Beijing Key Laboratory of Environmental & Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, China; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
Received: Mar. 7, 2018;       Accepted: Mar. 24, 2018;       Published: Apr. 12, 2018
DOI: 10.11648/j.ijbecs.20180401.15      View  867      Downloads  29
Abstract
The objective of the study was to investigate the role of CASC9 in esophageal cancer cells migration and invasion. Expression of long noncoding RNA (lncRNA) CASC9 was found high in esophageal cancer cells by qRT-PCR. The CASC9 knockdown effects on cell migration and invasion were analyzed in vitro. Expression of lncRNA CASC9 was found markedly high in KYSE-450 cell line as compared to other esophageal cancer cell lines. After the knockdown of CASC9, migration and invasion were significantly decreased as compared to negative control. Increased expression of CASC9 before knockdown and decreased expression after knockdown in esophageal cancer cells, showed that CASC9 may act as a biomarker for esophageal cancer and may help to understand the mechanisms of this lethal disease.
Keywords
CASC9, Esophageal Cancer, Cell Migration, Invasion, Long Noncoding RNA
To cite this article
Maliha Ghaffar, Sara Khodahemmati, Jintao Li, Muhammad Shahzad, Minglian Wang, Yangjunqi Wang, Yi Zeng, Down-Regulation of Long Non-Coding RNA CASC9 Suppresses the Migration and Invasion of Esophageal Cancer Cells, International Journal of Biomedical Engineering and Clinical Science. Vol. 4, No. 1, 2018, pp. 25-29. doi: 10.11648/j.ijbecs.20180401.15
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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